|
KMID : 0606920060140030143
|
|
Biomolecules & Therapeutics
2006 Volume.14 No. 3 p.143 ~ p.147
|
|
|
Induction of Nitric Oxide Production by Bafilomycin A1 in Mouse Leukemic Monocyte Cell Line
|
|
Hong Jang-Ja
Yasuhiro Nakano
Kazuo Ohuchi
Kang Young-Sook
|
|
|
Abstract
|
|
|
|
In the mouse leukemic monocyte cell line RAW 264.7, the vacuolar-type (H+)-ATPase (V-ATPase) inhibitor bafilomycin A1 at 10 and 100nM decreased cell growth and survival as determined by 3-(4,5-dime- thyl(thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in a concentration-dependent manner. At such concentrations, bafilomycin A1 induced nitric oxide (NO) production through the expression of inducible nitric oxide synthase (iNOS). The bafilomycin A1-induced NO production was inhibited by the NOS inhibitor NG-monomethyl-L-arginine acetate (L-NMMA). Our findings suggest that the V-ATPase inhibitor bafilomycin A1 induces NO production through the expression of iNOS protein.
|
|
|
KEYWORD
|
|
|
RAW 264.7 cells, Bafilomycin A1, Nitric oxide, V-ATPase inhibitor
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|